Evaluation – Questions & answers
[expand title=”Cite mechanisms that participate in the prevention of undesirable responses of the adaptive immune system.” tag=”strong”]Negative selection (or central tolerance), ignorance, peripheral tolerance by intrinsic (anergy) or extrinsic (Tregs) mechanisms.[/expand] [expand title=”Cite different mechanisms Tregs use to exert their suppressive functions.” tag=”strong”]
T cell deprivation of interleukin-2 by high CD25 expression, production of inhibitory cytokines (IL-10, TGFb, IL-35) or other molecules (adenosine), down-regulation of dendritic cell functions, cell killing.[/expand] [expand title=”What are the main 3 conclusions of Sakaguchi’s 1995 experiment in which transfer of CD4+CD25- T cells to an alymphocytic host provoked multiple autoimmune diseases that were prevented by CD25+ T cell addback?” tag=”strong”]
(1) a normal repertoire physiologically contains autoreactive T cells, (2) CD4+CD25+ T cells are a population of naturally occurring Tregs that control the activity of autoreactive T cells, and (3) Treg transfer might be used for therapy of autoimmune diseases.[/expand] [expand title=”What cytokine are Tregs critically dependent on?” tag=”strong”]
interleukin-2 (IL-2)[/expand] [expand title=”What phenotype can identify blood Tregs in patients using flow cytometry?” tag=”strong”]
CD4+ CD25hi ; CD4+ CD127-/lo ; CD4+ FOXP3+[/expand]
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