Murine model of coeliac disease
![DQ8-Dd-villin-IL-15tg mice were maintained on a gluten-free diet (GFD; sham), fed gluten for 60 days (gluten), or fed gluten for 30 days and then reverted to a GFD (gluten→GFD) for 30 days. a, Experimental timeline. b, Left, haematoxylin and eosin (H&E) staining of paraffin-embedded ileum sections. Scale bars, 200 μm. Right, graph depicts the ratio of the morphometric assessment of villus height to crypt depth (GFD, n = 9; gluten, n = 13; gluten→GFD, n = 11 mice). c, Serum levels of anti-DGP IgG antibodies were measured by ELISA. Serum was collected sequentially in the same mice (n = 12) before gluten feeding (untreated), 30 days after gluten feeding (gluten d30), and 30 days after reversion to a GFD (GFD d60). (Source Abadie et al., 2020)]()
Abadie et al., developed transgenic DQ8-Dd-villin-IL-15tg mice, which express the HLA-DQ8 [HLA associated with genetic predisposition to coeliac disease), as well as over-express IL-15 by both the lamina propria and…
ChelekaFebruary 27, 2020
